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Expand long template pcr system msds

2019-08-25 17:29

The exclusive global distribution agreement covers Roche Biochemical Reagent products, which include products for proteomics, cell analysis, and conventional PCR applications. These products complement SigmaAldrich's broad portfolio and allow customers to take advantage of SigmaAldrich's strong eCommerce and logistics capabilities.Specification Enzymes in the Expand Long Template PCR System were originally isolated from the thermophilic eubacteria Thermus aquaticus (Taq) BM and Thermococcus gorgonarius (Tgo), both expressed in E. coli. Enzyme acivities: Taq Polymerase: Highly processive 5'3' DNA polymerase, doublestrand specific 5'3' exonuclease, no 3'5' exonuclease activity expand long template pcr system msds

Expand Long Template PCR System is optimized to efficiently amplify large genomic DNA fragments around 20 kb long. Due to the inherent 35 exonuclease (proofreading) activity of Tgo DNA polymerase, Expand Long Template PCR System copies DNA threefold more accurately than Taq

Expand 20 kb PLUS 151 6 Expand Long Template, Expand 20 kb PLUS Bring Reliability and Consistency to your Long and Accurate PCR Expand Long Template simplies long and accurate PCR reactions. This optimized enzyme blend and threebuffer set produces PCR products from 5 to 20 kb in length. Even if Taq were able to amplify such Using the Expand Long Template PCR System, always thaw and equilibrate all buffers at 37 to 56 C before use. In addition, buffers should be vortexed thoroughly. When crystals have formed, incubate at 37 to 56 C until they are dissolved. After this step, do not place the buffers on ice. expand long template pcr system msds Mar 14, 2007  Should work, your amplified product contains a mix of with overhang and without overhang. We've succesfully TAcloned with expandhifi or expandlong template PCR products. If you don't get enough colonies, add regular taq at the end of your reaction and have it

up to 5 kb long GCRICH PCR System Amplification of Difficult Templates, protocol I, K, (page 103, 105) up to 5 kb long FastStart High Fidelity PCR System High Fidelity PCR, protocol E (page 83) Optimizing Multiplex PCR (Chapter 8) 525 kb long Expand Long Range dNTPack Long Template PCR, protocol G (page 94) PCR Protocol Selection Guide expand long template pcr system msds HawkZ05 Fast OneStep RTPCR Kit is supplied as a kit containing 2 vials of HawkZ05 Fast OneStep RTPCR Master Mix and 2 vials of RMS Manganese Acetate (25 mM). The master mix contains a reference dye (FRETROX) to run assays on realtime PCR instruments which require Rox for quantitative analysis. Expand High Fidelity PCR System is a mixture of Taq DNA polymerase and a DNA polymerase with proofreading activity for high yield and fidelity. It is optimized. Expand High FidelityPLUS PCR System is a mixture of Taq DNA polymerase and a to amplify a 4. 8 Figure 1: PCR amplification of long templates from nucleic acid samples obtained with the High Pure PCR Template Preparation Kit. Nucleic acids were prepared from blood or cultured human K562 cells. 250 ng of each preparation was amplified using a (tPA)specific primer and either the Expand Long Template PCR System Expand Long Template PCR System is optimized to efficiently amplify large genomic DNA fragments around 20 kb long. Due to the inherent 35 exonuclease (proofreading) activity of Tgo DNA polymerase, Expand Long Template PCR System copies DNA threefold more accurately than Taq

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