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Microfluidic cell culture systems for cellular analysis

2019-08-23 15:02

Oct 31, 2017 Microfluidics for Cell Culture and Analysis. From the initial vision of recreating entire chemistry laboratories on chips, microfluidics has evolved into niche applications for cell culture and analytical science. Microfluidics began in earnest during the late 1980s, when academic groups at the University of Delft (Netherlands), ETH (Zurich),Provides insights into the design and development of microfluidic systems with a specific focus on cell culture applications; Focuses on strategies and techniques for the design and fabrication of microfluidic systems and devices for cell culture; Provides balanced microfluidic cell culture systems for cellular analysis

We built a fully automated cell culture screening system based on a microfluidic chip that creates arbitrary culture media formulations in 96 independent culture chambers and maintains cell viability for weeks. Individual culture conditions are customized in terms of cell seeding density, composition of culture medium, and feeding schedule, and each chamber is imaged with timelapse microscopy.

Summary. NIST has taken advantage of recent technological advances to develop polymer microfluidic cell culture systems that provide a level of control over the cellular microenvironment unmatched in conventional cultures. Cell behavior is powerfully modulated by local extracellular cues such as: soluble signaling molecules and dissolved gases, Recently, several microfluidic systems have shown good realtime manipulation of cell culture and cellular responses to simultaneous stimulation of soluble cues (GmezSjberg et al. , 2007; Park et al. , 2010), and an excellent microfluidic system for studying mammalian cells in 3D microenvironments has presented one of its possible utilities microfluidic cell culture systems for cellular analysis The system, which allows online electrode cleaning and modification, was developed for realtime monitoring of cellular dynamics, exemplified in this work by monitoring of redox metabolism inside living yeast cells and dopamine release from PC12 cells. KEYWORDS: Microfluidic Cell Culture System, Online Measurement, Cellular Dynamics

microfluidic cell culture devices are introduced, and the developed microfluidic systems are described according to their cell type and target analytes. Furthermore, a suggestion concerning in vivolike microenvironment construction in a microfluidic cell culture system is addressed. Microfluidic Cell Culture Systems for Cellular Analysis microfluidic cell culture systems for cellular analysis In this paper, the microfluidic cell culture systems developed are reviewed and categorized according to their cell type and assay development. Apr 25, 2016  Microfluidic cell culture. Microfluidics has been used for cell biology studies as the dimensions of the microfluidic channels are well suited for the physical scale of cells. For example, eukaryotic cells have linear dimensions between m which falls within the range of Microfluidic cell culture is a promising technology for applications in the drug screening industry. Key benefits include improved biological function, higher quality cellbased data, reduced reagent consumption, and lower cost. In this work, we demonstrate how a microfluidic cell culture Microfluidics. Typically in microfluidic systems fluids are transported, mixed, separated or otherwise processed. The various applications of such systems rely on passive fluid control using capillary forces. In some applications, external actuation means are additionally used for a directed transport of the media.

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