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Gal 4 uas system

2019-08-19 22:10

Jul 21, 2017  The Gal4UAS System. At a more detailed level, the Gal4UAS system is a transcription activation system coopted from yeast. It is a Drosophila geneticists main workhorse to turn genes on or off. Gal4 is a transcriptional activator that binds to UAS enhancer sequences found in DNA.An example GAL4UAS system, with GAL4 lines and UAS reporter lines. The GAL4UAS system is a biochemical method used to study gene expression and function in organisms such as the fruit fly. It has also been adapted to study receptor chemicalbinding functions in vitro in cell culture. gal 4 uas system

GAL4UAS system The GAL4UAS system is a biochemical method used to study gene expression and function in organisms such as the fruit fly. It has also been adapted to study receptor chemicalbinding functions in vitro in cell culture. It was developed by Hitoshi Kakidani and Mark Ptashne, and Nicholas Webster and Pierre Chambon in 1988, then adapted by Andrea Brand and Norbert Perrimon in 1993

The Gal4UAS system was initially employed as a twocomponent gene expression system in Drosophila (Brand& Perrimon, 1993). Specifically, fly lines expressing Gal4 in specific tissues and cells and a fly line carrying the lacZ gene downstream of UAS were created and crossed. gal 4 uas system

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